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KMID : 1024520140230010089
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Journal of the Environmental Sciences
2014 Volume.23 No. 1 p.89 ~ p.96
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Screening of Biological Activity of Solvent Extract from Styela clava Tunic for Fishery Waste Recycling
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Lee Sang-Mi
Kang Eun-Jin
Go Tae-Hun
Jeong Seong-Yun
Park Geun-Tae
Lee Hee-Sup
Hwang Dae-Yean
Jung Young-Jin
Son Hong-Joo
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Abstract
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Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental
problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration (IC50) against tyrosinase activity was 0.36×104 mg/ml in ethanol extract, 0.11×103 mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract.
Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts.Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.
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KEYWORD
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Antioxidant, Solvent extract, Styela clava, Whitening effect
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